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Activity assay for glucose dehydrogenase The activity of Bs GDH from Bacillus subtilis (0.041 mg/mL) was assessed at 580 nm using glucose (6 mM) and NADP + (0.004 mM) as substrates, nitrotetrazolium blue chloride (NBT) (0.2 mg/mL) and phenazine methosulfate (PMS) (0.01 mg/mL) were added to the assay as they react with the NADPH produced by the GDH to produce an insoluble blue-purple formazan which can be measured at 580 nm 42

GR is the only enzyme which is a flavoprotein that catalyses the reduction of oxidized glutathione to reduced glutathione

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Atherosclerosis is a type of cardiovascular disease characterized by lipid deposition and chronic inflammatory response in blood vessel walls, and is the primary pathological basis of cardiovascular and cerebrovascular events such as myocardial infarction and stroke (99)

28.2% in controls) Improved collagen organization and increased deposition Enhanced glutathione levels and antioxidant enzyme activity Increased angiogenesis through VEGF and FGF-2 stimulation Reduced pro-inflammatory markers (TNF-, IL-6) with decreased NF-B signaling Fibroblast migration stimulation and extracellular matrix remodeling GHK-Cu research consistently demonstrates that the peptide is biologically active at very low concentrations in the nanomolar range (110 nM)

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